Isolation and Growth Characterization of the Fresh Water Algae Chlorosarcinopsis Eremi on Different Growth Media | Zendy

Dhanalakshmi Marimuthu | Zendy; Jayaraman Angayarkanni | Zendy

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Isolation and Growth Characterization of the Fresh Water Algae Chlorosarcinopsis Eremi on Different Growth Media

Author(s) -

Dhanalakshmi Marimuthu,

Jayaraman Angayarkanni

Publication year - 2018

Publication title -

journal of pure and applied microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.149

H-Index - 16

eISSN - 2581-690X

pISSN - 0973-7510

DOI - 10.22207/jpam.12.1.46

Subject(s) - isolation (microbiology) , algae , characterization (materials science) , chemistry , botany , biology , materials science , microbiology and biotechnology , nanotechnology

In the present study the freshwater algae Chlorosarcinopsis eremi was isolated from the water sample of Kovai Kutralum. The pure culture of the algae was obtained using serial dilution technique followed by streak plate method. The species was observed under light microscopy and identified with the help of algal manuals and also by sequencing 18S rRNA genes. The growth of the algae, C. eremi was characterized in different growth media. The different growth media’s include Bold’s Basal medium, Modified Bourrelly medium, BG 11 medium, Half strength Chu10 medium and Modified Hoagland’s medium. The growth was measured by observed optical density of the biomass and chlorophyll “a” content. The experiment was carried out three times and mean values and standard deviation were calculated. From the results it is concluded that growth of C. eremi was maximum in Bold’s basal medium when compared with other media tested.

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