Open AccessEvaluation of Diagnostic Potential of Echinococcus granulosus Recombinant EgAgB8/1, EgAgB8/2 and EPC1 Antigens for Cystic Echinococcosis in Goats
Author(s) -
Ajayta Rialch,
Mary Nisha Tigga,
A. B. Arun,
S. Samanta,
O.K. Raina
Publication year - 2017
Publication title -
journal of pure and applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.149
H-Index - 16
eISSN - 2581-690X
pISSN - 0973-7510
DOI - 10.22207/jpam.11.1.58
Subject(s) - echinococcus granulosus , echinococcosis , cystic echinococcosis , antigen , cestode infections , biology , echinococcus , recombinant dna , helminths , helminthiasis , virology , veterinary medicine , microbiology and biotechnology , immunology , medicine , zoology , gene , biochemistry
Echinococcus granulosus recombinant proteins including two antigen B subunits EgAgB8/1 and EgAgB8/2 and Echinococcus protoscolex calcium binding protein (EPC1) were expressed in prokaryotic expression vectors. The diagnostic potential of these three recombinant proteins was evaluated in the detection of cystic echinococcosis in goats in IgG-ELISA. The EgAgB8/1 and EgAgB8/2 recombinant proteins reacted fairly with the hydatid infected goats with sensitivity of 66.7% and 80.0% and specificity of 71.3% and 73.3%, respectively while EPC1 recombinant protein showed lower sensitivity (60%) but comparable specificity (72.3%). Cross-reactivity of these three antigens with goat gastro-intestinal strongyle nematodes and Taenia hydatigena under field conditions was studied. Results showed that EgAgB8/1, EgAgB8/2 and EPC1 antigens cross-reacted with most of the parasites in the goat host.
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