Col2CreERT2, a mouse model for a chondrocyte-specific and inducible gene deletion | Zendy

Michael Chen | Zendy; Siwei Li | Zendy; Wanqing Xie | Zendy; B Wang | Zendy; D Chen | Zendy

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Col2CreERT2, a mouse model for a chondrocyte-specific and inducible gene deletion

Author(s) -

Michael Chen,

Siwei Li,

Wanqing Xie,

B Wang,

D Chen

Publication year - 2014

Publication title -

european cells and materials

Language(s) - English

Resource type - Journals

ISSN - 1473-2262

DOI - 10.22203/ecm.v028a16

Subject(s) - chondrocyte , cartilage , osteoarthritis , intervertebral disc , gene , fusion gene , animal model , biology , temporomandibular joint , pathology , degeneration (medical) , microbiology and biotechnology , medicine , bioinformatics , anatomy , genetics , endocrinology , alternative medicine

In 2007 and 2008, we published two articles reporting a tamoxifen (TM)-inducible, chondrocyte-specific gene-targeting mouse model in which the expression of CreER(T2) is driven by the type II collagen promoter (Col2CreER(T2)). The fusion protein is specifically expressed and translocated into the nucleus upon TM administration, which in turn triggers gene recombination. Since then, this animal model has become a powerful tool to study the molecular mechanism of skeletal development and degenerative cartilage diseases, including knee joint osteoarthritis (OA), temporomandibular joint (TMJ) OA, and intervertebral disc (IVD) degeneration. In this review article, we summarise the application of Col2CreER(T2) mice and discuss the potential usage of this animal model in a broad spectrum of cartilage development and molecular pathology studies.

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