KONSENTRASI HAMBAT MINIMUM (KHM) EKSTRAK DAUN TEH (Camellia sinensis L.) PADA Escherichia coli DAN Salmonella sp

Diarrhea is the condition loosing too much liquid and electrolyte through feces and it is second biggest cause of infant death in Indonesia. Diarrhea is caused by Salmonella sp and Escherichia coli bacteria. Inappropriate drug used such as anti diarrhea and antibiotic can cause another health problem. An alternative treatment which does not cause side effect to the diarrhea victim is using an anti microbe active of the plant medicine. One of the plant which is used by the people as a treatment for the diarrhea victim is tea leaves (Camellia sinensis L.). it contains anti microbe active such as catecin, tannin, flavonoid and saponin. Thus, the research was conducted to find out the minimum restriction concentrate of Camellia sinensis L. in E. coli and Salmonella sp. The minimum restriction concentration is used dilution method with jelly as the media (Jelly dilution method) by double it. The concentrations of E. coli are 50 %, 25 %, 12,5 %, 6,25 %, 3,125 % and 1,56 % with 10 % antibiotic ampicilin control. The concentrations for Salmonella sp are 50 %, 25 %, 12,5 %, 6,25 %, 3,125 %, 1,56 %, 0,78 %, 0,39 %, 0,195 % and 0,0975 %. The data was analyzed in qualitative method by examining the minimum restriction of tea leaves extract (Camellia sinensis L.) in the bacteria that caused diarrhea. The research was conducted on August 2010 in Biology Laboratory of STKIP PGRI West Sumatera. Tea leaves was extracted in Chemical Laboratory of Kopertis Wilayah X Padang. The research found that E. coli is more resistant to tea leaves than Salmonella sp. The minimum restriction concentrate of tea leaves extract (Camellia sinensis L.) in E. coli is 3,125% and 0,0975% in Salmonella sp.