Open AccessImpact of Fetal Size on Myogenesis and Pax7+ Progenitor Cell Populations
Author(s) -
M. A. Vaughn,
Jodi M Morton,
J. A. Feldpausch,
K. J. Phelps,
J. A. Noel,
D. L. Davis,
J. L. Nelssen,
J. M. Gonzalez
Publication year - 2017
Publication title -
meat and muscle biology
Language(s) - English
Resource type - Journals
ISSN - 2575-985X
DOI - 10.22175/rmc2016.133
Subject(s) - fetus , anatomy , myogenesis , gestation , biology , skeletal muscle , progenitor cell , andrology , medicine , pregnancy , stem cell , genetics
In experiment 1, gravid gilts (n = 36; PIC 1050 × 327) were harvested at d-60 of gestation and the smallest (SM), median (ME), and largest (LG) male fetuses were removed for muscle morphometric analysis based on crown-to-rump length. In experiment 2, gravid gilts (n = 24) were harvested at d-90 of gestation and fetal weight was used to categorize SM, ME, and LG fetuses. Whole muscle cross-sectional area (CSA) of the Longissimus muscle of each fetus was measured at the fifth rib and first lumbar vertebrae. Tissue samples corresponding to each location were cryopreserved, cut into 10 μm sections, and subjected to immunohistochemistry to identify primary and secondary muscle fibers. Muscle fiber number, CSA, and number of Pax7+ progenitor cells were determined.
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