Prevalence of carbapenemase-producing organisms at the Kidney Center of Rawalpindi (Pakistan) and evaluation of an advanced molecular microarray-based carbapenemase assay | Zendy

Sascha D. Braun | Zendy; Bushra Jamil | Zendy; Muhammad Ali Syed | Zendy; Shahid Ahmad Abbasi | Zendy; Daniel L. Weiß | Zendy; Peter Slickers | Zendy; Stefan Monecke | Zendy; Ines Engelmann | Zendy; Ralf Ehricht | Zendy

Open Access

Prevalence of carbapenemase-producing organisms at the Kidney Center of Rawalpindi (Pakistan) and evaluation of an advanced molecular microarray-based carbapenemase assay

Author(s) -

Sascha D. Braun,

Bushra Jamil,

Muhammad Ali Syed,

Shahid Ahmad Abbasi,

Daniel L. Weiß,

Peter Slickers,

Stefan Monecke,

Ines Engelmann,

Ralf Ehricht

Publication year - 2018

Publication title -

future microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.797

H-Index - 82

eISSN - 1746-0921

pISSN - 1746-0913

DOI - 10.2217/fmb-2018-0082

Subject(s) - microbiology and biotechnology , biology , microarray , medicine , virology , computational biology , gene , genetics , gene expression

Aim: A DNA microarray-based assay for the detection of antimicrobial resistance (AMR) genes was used to study carbapenemase-producing organisms at the Kidney Center of Rawalpindi, Pakistan. Methods: The evaluation of this assay was performed using 97 reference strains with confirmed AMR genes. Testing of 7857 clinical samples identified 425 Gram-negative bacteria out of which 82 appeared carbapenem resistant. These isolates were analyzed using VITEK-2 for phenotyping and the described AMR assay for genotyping. Results: The most prevalent carbapenemase gene was blaNDM and in 12 isolates we detected two carbapenemase genes (e.g., blaNDM/blaOXA-48). Conclusion: Our prevalence data from Pakistan show that – as in other parts of the world – carbapenemase-producing organisms with different underlying resistance mechanisms are emerging, and this warrants intensified and constant surveillance.

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