Isobaric tags for relative and absolute quantitation proteomics analysis of gene regulation by SprC in Staphylococcus aureus | Zendy

Huanqiang Zhao | Zendy; Fupin Hu | Zendy; Yang Han | Zendy; Baixing Ding | Zendy; Xiaogang Xu | Zendy; Chunyan He | Zendy; Zelin Cui | Zendy; Wen Shu | Zendy; Qingzhong Liu | Zendy

Open Access

Isobaric tags for relative and absolute quantitation proteomics analysis of gene regulation by SprC in Staphylococcus aureus

Author(s) -

Huanqiang Zhao,

Fupin Hu,

Yang Han,

Baixing Ding,

Xiaogang Xu,

Chunyan He,

Zelin Cui,

Wen Shu,

Qingzhong Liu

Publication year - 2017

Publication title -

future microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.797

H-Index - 82

eISSN - 1746-0921

pISSN - 1746-0913

DOI - 10.2217/fmb-2017-0033

Subject(s) - staphylococcus aureus , isobaric process , gene , proteomics , microbiology and biotechnology , isobaric labeling , quantitative proteomics , biology , computational biology , bacteria , genetics , physics , thermodynamics

Aim: To explore the complete gene networks regulated by small RNA SprC and its targets in Staphylococcus aureus. Materials & methods: The isobaric tags for relative and absolute quantitation and bioinformatic methods were utilized to identify and analyze the target proteins affected by SprC in S. aureus N315. Results: Proteomic analysis showed that the expression of 44 proteins was modulated by SprC. Further, bioinformatic analysis displayed that these affected proteins mainly associated with metabolic and cellular process, biological regulation and catalytic activity. Conclusion: Our data provide a rich resource of SprC targets in S. aureus, although the mechanism of regulation by SprC is yet to be elucidated.

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