Generation of Definitive Endoderm from Human Embryonic Stem Cells Cultured in Feeder Layer-Free Conditions | Zendy

Charles C. King | Zendy; Gillian M. Beattie | Zendy; Ana D. Lopez | Zendy; Alberto Hayek | Zendy

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Generation of Definitive Endoderm from Human Embryonic Stem Cells Cultured in Feeder Layer-Free Conditions

Author(s) -

Charles C. King,

Gillian M. Beattie,

Ana D. Lopez,

Alberto Hayek

Publication year - 2008

Publication title -

regenerative medicine

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.572

H-Index - 63

eISSN - 1746-076X

pISSN - 1746-0751

DOI - 10.2217/17460751.3.2.175

Subject(s) - endoderm , embryonic stem cell , biology , microbiology and biotechnology , cell culture , sialic acid , cellular differentiation , stem cell , cell sorting , chemistry , in vitro , biochemistry , genetics , gene

The purpose of these studies was twofold: to reduce the level of nonhuman, potentially immunogenic sialic acid N-glycolylneuraminic acid (Neu5Gc) in human embryonic stem cells (hESCs) through culture of the cells in the absence of feeder layers; and to determine whether directed differentiation was preserved under these conditions, that is, using exclusively human-derived products.

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