Open AccessMass Spectrometry–Based Proteomic Diagnosis of Renal Immunoglobulin Heavy Chain Amyloidosis
Author(s) -
Sanjeev Sethi,
Jason D. Theis,
Nelson Leung,
Angela Dispenzieri,
Samih H. Nasr,
Mary E. Fidler,
Lynn D. Cornell,
Jeffrey D. Gamez,
Julie A. Vrana,
Ahmet Doğan
Publication year - 2010
Publication title -
clinical journal of the american society of nephrology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.755
H-Index - 151
eISSN - 1555-905X
pISSN - 1555-9041
DOI - 10.2215/cjn.02890310
Subject(s) - immunoglobulin light chain , amyloidosis , transthyretin , amyloid (mycology) , al amyloidosis , laser capture microdissection , pathology , medicine , heavy chain , antibody , chemistry , biochemistry , immunology , gene , gene expression
Amyloidosis is a group of disorders characterized by accumulation of extracellular deposition of proteins as insoluble aggregates. The clinical management of amyloidosis is based on identifying the underlying etiology and accurate typing of the amyloid. Ig heavy chain amyloid involving the kidney is poorly recognized and often poses a diagnostic dilemma. DESIGN, SETTING, PARTICIPANTS, & MEASURES: In this study, we describe the use of laser microdissection (LMD) and mass spectrometry (MS)-based proteomic analysis for the accurate typing of 14 cases of amyloidosis. We also describe the clinicopathologic findings of four problematic cases of renal Ig heavy chain amyloidosis that required LMD/MS proteomic analysis for accurate typing of the amyloid.