POLY(GLYCIDYL METHACRYLATE-DIVINYLBENZENE) MONOLITHIC CAPILLARY AS A STATIONARY PHASE FOR THE REVERSED-PHASE CHROMATOGRAPHIC SEPARATION OF PROTEINS | Zendy

Nurul Hidayat Aprilita | Zendy; Rania Bakry | Zendy; Christian W. Huck | Zendy; Guenther K. Bonn | Zendy

Open Access

POLY(GLYCIDYL METHACRYLATE-DIVINYLBENZENE) MONOLITHIC CAPILLARY AS A STATIONARY PHASE FOR THE REVERSED-PHASE CHROMATOGRAPHIC SEPARATION OF PROTEINS

Author(s) -

Nurul Hidayat Aprilita,

Rania Bakry,

Christian W. Huck,

Guenther K. Bonn

Publication year - 2010

Publication title -

indonesian journal of chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.273

H-Index - 14

eISSN - 2460-1578

pISSN - 1411-9420

DOI - 10.22146/ijc.21830

Subject(s) - divinylbenzene , glycidyl methacrylate , monolith , chemistry , van deemter equation , capillary action , chromatography , monolithic hplc column , methacrylate , capillary electrochromatography , polymerization , tetrahydrofuran , phase (matter) , stationary phase , analytical chemistry (journal) , styrene , polymer , organic chemistry , copolymer , materials science , composite material , catalysis , solvent

Capillary column with monolithic stationary phase was prepared from silanized fused-silica capillary of 200 µm I.D. by in situ free radical polymerization of divinylbenzene with glycidy methacrylate in the presence of decanol and tetrahydrofuran as porogens. The hydrodynamic and chromatographic properties of this monolith, such as backpressure at different flow-rate, pore size distribution, van Deemter plot and the effect of varying gradient-rate were investigated. Poly(glycidyl methacrylate-divinylbenzene) monolithic capillary has been used successfully for the reversed-phase chromatographic separation of proteins.

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