IMMOBILIZATION OF PAPAIN ON CHITOSAN | Zendy

Sari Edi Cahyaningrum | Zendy; Narsito Narsito | Zendy; Sri Juari Santoso | Zendy; Rudiana Agustini | Zendy

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IMMOBILIZATION OF PAPAIN ON CHITOSAN

Author(s) -

Sari Edi Cahyaningrum,

Narsito Narsito,

Sri Juari Santoso,

Rudiana Agustini

Publication year - 2010

Publication title -

indonesian journal of chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.273

H-Index - 14

eISSN - 2460-1578

pISSN - 1411-9420

DOI - 10.22146/ijc.21609

Subject(s) - papain , chemistry , chitosan , thermal stability , chromatography , enzyme , organic chemistry

In this study, papain was immobilized on chitosan with Mg(II) cosslinked agent. Studies on free and immobilized papain systems for determination of optimum pH, optimum temperatur, thermal stability and reusability were carried out. The results showed that free papain had optimum pH 6.5 and optimum temperature 55 °C while the immobile papain had optimum pH 8 and optimum temperature 80 °C. The thermal stability of the immobilized papain, relative to that of the free papain, was markedly increased. The immobilized papain can be reused for at least six times.

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