Open AccessTHE POTENTIOMETRIC UREA BIOSENSOR USING CHITOSAN MEMBRANE
Author(s) -
Ani Mulyasuryani,
Anna Roosdiana,
Arie Srihardyastutie
Publication year - 2010
Publication title -
indonesian journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.273
H-Index - 14
eISSN - 2460-1578
pISSN - 1411-9420
DOI - 10.22146/ijc.21454
Subject(s) - biosensor , chemistry , urease , potentiometric titration , chitosan , urea , membrane , hydrolysis , chromatography , detection limit , nuclear chemistry , electrode , biochemistry
Potentiometric urea biosensor development is based on urea hydrolysis by urease resulted CO2. The biosensor is used chitosan membrane and the H3O + electrode as a transducer. The research was studied of effecting pH and membrane thickness to the biosensor performance. The best biosensor performance resulted at pH 7.3 and membrane thickness of 0.2 mm. The biosensor has a Nerntian factor of 28.47 mV/decade; the concentration range is 0.1 up to 6.00 ppm; and the limit of detection is 0.073 ppm. The response time of this biosensor is 280 seconds, efficiency 32 samples and accuracy 94% up to 99%.
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