Glucose Oxidase Immobilization on TMAH-Modified Bentonite | Zendy

Ruth Chrisnasari | Zendy; Zerlina Gabriela Wuisan | Zendy; Arief Budhyantoro | Zendy; Restu Kartiko Widi | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Glucose Oxidase Immobilization on TMAH-Modified Bentonite

Author(s) -

Ruth Chrisnasari,

Zerlina Gabriela Wuisan,

Arief Budhyantoro,

Restu Kartiko Widi

Publication year - 2015

Publication title -

indonesian journal of chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.273

H-Index - 14

eISSN - 2460-1578

pISSN - 1411-9420

DOI - 10.22146/ijc.21219

Subject(s) - chemistry , bentonite , fourier transform infrared spectroscopy , ammonium hydroxide , glucose oxidase , nuclear chemistry , immobilized enzyme , ammonium , chromatography , chemical engineering , enzyme , inorganic chemistry , organic chemistry , engineering

The influence of bentonite modification by tetramethyl ammonium hydroxide (TMAH) on its capability to immobilize glucose oxidase (GOX) was studied. Modification of bentonite was conducted by the adding of 0-5% (v/v) TMAH. The observed results show that the different concentrations of TMAH affect the percentage of immobilized enzyme. The results of this study show that the best concentration of TMAH is 5% (v/v) which can immobilize up to 84.71% of GOX. X-ray diffraction (XRD) and Fourier Transforms Infrared Spectroscopy (FTIR) studies have been carried out to observe the structural changes in bentonite due to TMAH modification. The obtained immobilized GOX show the optimum catalytic activity on reaction temperature of 40-50 °C and pH of 7. The immobilized GOX kinetics at the optimum conditions determined the K m and V max value to be 4.96x10 -2 mM and 4.99x10 -3 mM.min -1 respectively. In addition, the immobilized GOX on TMAH-modified bentonite is stable enough so it could be re-used six times before its activity decreased by 39.44%.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research