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This study aimed to determine phenolic compounds of Artemisia spicigera (family Asteraceae) growing in East-Azarbaijan province of Iran. 20%, 40 % and 60% SPE fractions of methanolic extract of A. spicigera, were subjected to reversed phase preparative HPLC, with the mobile phase consisted of methanol and water. Structural identification of phytochemicals by spectroscopic methods including UV and NMR spectroscopy, yielded 4, 6-di methoxy acetophenone-2-O-β-D-glucopyranoside from 20%, 5-methoxyluteolin 7-O-β-D-glucopyranoside, luteolin and chrysoeriol 7-O-β-D-glucopyranoside from 40% and 5-methoxy luteolin from 60% SPE fractions. Although within identified pure compounds, luteolin was the only phenolic reported from some other species of Artemisia, but occurrence of remained identified phenolics in this study, was firstly reported from Artemisia genus. Further phytochemical investigations were proposed in order to isolate some other active fractions and pure compounds.

Phenolic Derivatives of Artemisia Spicigera C. Koch Growing in Iran