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This research evaluated the insecticidal activity of various native isolates of Bacillus thuringiensis against Spodoptera frugiperda, From Soils of Funza and Silvania (Cundinamarca) Tunja (Boyaca) in crops of beans, peas, maize and orange compared with a commercial witness HD1 (subsp. Kurstaki) against S. frugiperda. LC50 lethal concentration average was initially determined by the "probit" method, which was 5.88 E- 4 mg ai / ml of diet, strain HD1 with different doses (0.0; 6.8 E-2; 3.4E-2 ; 6.8E-3; 6.8E-4; 6.8E-5 6.8E-6 and ai mg / ml diet) in first instar larvae S. frugiperda. In addition to qualitative observations mortality was measured at 96 and 120 hours. Subsequently the determined median lethal concentration (LC50) with strain HD1 proceeded to use this concentration in each native B. thuringiensis isolates to be compared with the commercial control CL50. Design was completely randomized with 7 treatments (each with 25 first instar larvae) and 3 replications. For the second test determining promissory isolates of Bt, a completely randomized design with 11 treatments (each with 20 first instar larvae) and 3 replications was arranged. Results indicated that M3008 and M4001 native isolates have higher mortality averages, which were 75% and 71.65% respectively in first instar larvae in purified diet, compared with mortality LC50 of commercial control. Key words: Bioassay, fungi, larvae, entomopathogenic, mortality.

Acción de cepas nativas de Bacillus thuringiensis (Berliner), como control biológico de Spodoptera frugiperda (J.E. Smith). Lepidoptera: Noctuidae