Development of Solid Phase Microextraction for Determination of Carbon tetrachloride and Chloroform in Air by Gas Chromatography-Mass Spectrometry

In this research Solid Phase Micro Extraction )SPME( for sampling and determination of carbon tetrachloride and chloroform in air was developed and the effect of ambient parameters on SPME was assessed. Methods: For this purpose, a standard chamber was built in the laboratory. The concentrations of compounds in the chamber were measured with SPME. The optimum condition for extraction was determined and compared with 1003 method of national institute occupational safety and health )NIOSH-1003(. The samples were analyzed with gas chromatography-mass spectrometry. Results: Carboxen-Polydimethylsiloxane )CAR/PDMS( has high adsorption compared with other fibers and there was no significant difference between sampling rates at different temperatures )range of 20 to 30 °C( and air velocities )2 to 50 cm/s(, yet Relative Humidity )RH( had a significant effect on sampling rate. The results showed that samples can be stored in a refrigerator at 4°C for three days. The correlation coefficient of results between SPME and NIOSH-1003 for carbon tetrachloride and chloroform were 0.99 and 0.98, respectively and relative standard deviation of reproducibility between fibers for carbon tetrachloride and chloroform were 13.6 and 12.8, respectively. Conclusions: This study showed that SPME was more sensitive than the NIOSH-1003 method, and was successfully applied for determination of time weight average of carbon tetrachloride and chloroform as a passive method. Received: 06.04.2016 Accepted: 15.05.2016