Ethanol Production From Cassava Wastes (Pulp And Peel) Using Alcohol Tolerant Yeast Isolated From Palm Wine
Author(s) -
E. J. Archibong,
Ifeanyi Emmanuel Obika,
Onyedika I. Okafor,
Uchechi-Ben Okafor,
C S Ezewuzie,
Chinyere Constance Ezemba,
N C Awah,
Benedict C. Okeke,
Chikodili Gladys Anaukwu,
V. N. Anakwenze
Publication year - 2016
Publication title -
american journal of life science researches
Language(s) - English
Resource type - Journals
eISSN - 2375-7485
pISSN - 2332-0206
DOI - 10.21859/ajlsr-040305
Subject(s) - fermentation , pulp (tooth) , ethanol fuel , ethanol , food science , chemistry , wine , starch , amylase , ethanol fermentation , alcohol , yeast , hydrolysis , alpha amylase , banana peel , enzyme , biochemistry , medicine , pathology
The efficient ethanol production using Saccharomyces spp. isolated from palm wine in utilizing industrial wastes (cassava pulp and peel) was studied in the liquid state fermentation process. Methods: The percentage alcohol in the cassava pulp and peel was obtained by digesting the cassava waste with three different commercial exogenous enzymes which include alpha amylase (Termamyl), Amyloglucosidase (AMG), β-glucanase t a-amylase (Cereflo) and fermenting with yeast ( Saccharomyces spp.) isolated from palm wine. The combination of two enzymes in starch hydrolysis produced more sugars than individual enzyme usage and also yielded more of ethanol when compared to a single enzyme activity. It was also observed that AMG combined with Termamyl yielded 2.05% ethanol. Cereflo combined with Termamyl yielded 1.6% ethanol while Tennamyl alone yielded 1.26% ethanol from cassava pulp. However, it was also observed that AMG combined with Termamyl yielded 0.46% ethanol, Cereflo combined with Termamyl yielded 0.73% ethanol while Termamyl alone yielded 0.33% ethanol from cassava peel. Results: This implies that ethanol produced from cassava pulp is higher than ethanol produced from cassava peel, since the cassava pulp contains high starch than the peel. Conclusions: These digestions using these enzymes and subsequent ethanol production can go a long way in waste management for economic purposes.
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