Optimizing the pure genomic DNA isolation procedure for Plectranthus amboinicus DNA – A prerequisite for further genomic Studies | Zendy

S. Manikandan | Zendy; S. Ansarali | Zendy; Ganapathy Murugan Alagu Lakshmanan | Zendy

Open Access

Optimizing the pure genomic DNA isolation procedure for Plectranthus amboinicus DNA – A prerequisite for further genomic Studies

Author(s) -

S. Manikandan,

S. Ansarali,

Ganapathy Murugan Alagu Lakshmanan

Publication year - 2017

Publication title -

journal of applied and advanced research

Language(s) - English

Resource type - Journals

ISSN - 2519-9412

DOI - 10.21839/jaar.2017.v2i4.97

Subject(s) - genomic dna , dna , biology , dna barcoding , dna extraction , isolation (microbiology) , genetics , polymerase chain reaction , gene , bioinformatics , evolutionary biology

The DNA isolation procedure for different plant groups have been studied and standardized. The isolation of pure genomic DNA is the most essential component for any type of molecular studies. The present work is aimed to identify suitable DNA markers for the amplification of P.amboinicus DNA becomes a great hurdle for DNA barcoding studies carried out by rbcL and matK primers Used in the members of Lamiaceae. To solve this problem, The DNA was extracted by three methods from fresh young leaf tissue of P.amboinicus . After the evaluationthe outcome of these methods, one most suitable modified method was selected for isolating DNA from young leaves of P.amboinicus and selected for suitable DNA barcoding markers for PCR amplification. The quality and quantity of DNAs are a prerequisite for genetic studies for a variety of plants including P.amboinicus . The quantity and quality of the DNA extracted by this method wasused for suitable DNA barcoding markers selection.

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