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2 ABSTRAK Salah satu mekanisme yang menerangkan patogenesis hipertensi pada obesitas adalah aktifasi sistem renin angiotensin. Angiotensinogen merupakan prekursor angiotensin II yang berperan dalam patofisiologi hipertensi. Ekspresi angiotensinogen dipengaruhi oleh kondisi lingkungan di sekitarnya. Penelitian sebelumnya secara in vivo memperlihatkan bahwa hiperglikemia dapat memodulasi ekspresi gen angiotensinogen di jaringan adiposa. Tujuan penelitian ini adalah ingin mengetahui apakah terjadi peningkatan sekresi angiotensinogen pada kultur sel adiposit yang di papar glukosa suprafisiologis selama 24 jam. Kultur sel adiposit diisolasi dari jaringan adiposa viseral tikus Rattus Novergicus strain Wistar jantan berusia 2-3 minggu. Kultur sel adiposit dibagi dalam 3 perlakuan, dipapar glukosa dengan konsentrasi 5 mM (sebagai kondisi fisiologis), 11 mM dan 25 mM sebagai kondisi glukosa tinggi, selama 24 jam. Dilakukan pengamatan kadar angiotensinogen pada medium kultur menggunakan ELISA. Hasil penelitian menunjukkan terdapat peningkatan yang signifikan kadar angiotensinogen pada medium kultur sel adiposit yang dipapar glukosa 25 mM dibandingkan pada paparan glukosa 5 mM (p=0,000) dan 11 mM (p=0,002). Paparan glukosa tinggi (25 mM) selama 24 jam dapat meningkatkan sekresi angiotensinogen pada kultur sel adiposit. Kata Kunci: Angiotensinogen, glukosa suprafisiologis, kultur adiposit ABSTRACT One mechanism that explains the pathogenesis hypertension in obesity is the activation of renin-angiotensin system. Angiotensinogen is precursor of angiotensin II, which plays an important role in pathophysiology of hypertension. Angiotensinogen expression level in adipocyte is influenced by the condition of their environment. Previous research showed that hyperglycemia can modulate angiotensinogen gene expression in adipose tissue. The purpose of this research was to know whether acute (24 hours) supraphysiologic glucose exposure can elevate angiotensinogen secretion in adipocyte culture. There were adipocytes cultures isolated from adipose viseral tissue of Rattus Novergicus rats (age 2-3 weeks), exposed with 5 mM glucose (as physiological condition), 11 mM and 25 mM glucose for 24 hours. Detection of angiotensinogen concentrations in adipocyte culture medium was performed by ELISA. The results showed that angiotensinogen secretion in adipocyte culture exposed with 25 mM is significantly higher than those of 5 mM glucose (p=0,000) and 11 mM glucose (p=0,002). It was concluded that the exposure of adipocyte with high glucose (25 mM) in 24 hours increases the secretion of angiotensinogen.

Peningkatan Sekresi Angiotensinogen pada Kultur Sel Adiposit akibat Paparan Glukosa Suprafisologis secara Akut