Open AccessPreparation and Manipulation of Olfactory Epithelium Explant Cultures for Measurement of the Mechanical Tension of Individual Axons Using the Biomembrane Force Probe
Author(s) -
Isabelle Dusart,
Alain Trembleau
Publication year - 2019
Publication title -
bio-protocol
Language(s) - English
Resource type - Journals
ISSN - 2331-8325
DOI - 10.21769/bioprotoc.3213
Subject(s) - explant culture , ex vivo , tension (geology) , anatomy , olfactory system , biology , biomedical engineering , microbiology and biotechnology , in vivo , neuroscience , materials science , in vitro , medicine , biochemistry , ultimate tensile strength , metallurgy
In this paper, we describe a protocol allowing measurement of the mechanical tension of individual axons grown ex vivo from neural tissue explants. This protocol was developed with primary cultures of olfactory epithelium explants from embryonic (E13.5) mice. It includes a detailed description of explant dissection and culture, as well as the main steps of the procedure for axon tension measurement using the previously established Biomembrane Force Probe.