Oral Microbiome Characterization in Murine Models | Zendy

Loreto Abusleme | Zendy; BoYoung Hong | Zendy; Anilei Hoare | Zendy; Joanne E. Konkel | Zendy; Patricia I. Diaz | Zendy; Niki M. Moutsopoulos | Zendy

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Oral Microbiome Characterization in Murine Models

Author(s) -

Loreto Abusleme,

BoYoung Hong,

Anilei Hoare,

Joanne E. Konkel,

Patricia I. Diaz,

Niki M. Moutsopoulos

Publication year - 2017

Publication title -

bio-protocol

Language(s) - English

Resource type - Journals

ISSN - 2331-8325

DOI - 10.21769/bioprotoc.2655

Subject(s) - microbiome , oral microbiome , periodontitis , oral cavity , biology , immune system , immunology , periodontal disease , computational biology , metagenomics , medicine , gene , bioinformatics , genetics , dentistry

The oral microbiome has been implicated as a trigger for immune responsiveness in the oral cavity, particularly in the setting of the inflammatory disease periodontitis. The protocol presented here is aimed at characterizing the oral microbiome in murine models at steady state and during perturbations of immunity or physiology. Herein, we describe murine oral microbiome sampling procedures, processing of low biomass samples and subsequent microbiome characterization based on 16S rRNA gene sequencing.

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