Open AccessGene Dosage Experiments in Enterobacteriaceae Using Arabinose-regulated Promoters
Author(s) -
Sanchari Bhattacharyya,
Shimon Bershtein,
Eugene I. Shakhnovich
Publication year - 2017
Publication title -
bio-protocol
Language(s) - English
Resource type - Journals
ISSN - 2331-8325
DOI - 10.21769/bioprotoc.2396
Subject(s) - arabinose , plasmid , western blot , enterobacteriaceae , gene , promoter , escherichia coli , gene expression , biology , microbiology and biotechnology , protein expression , intracellular , biochemistry , xylose , fermentation
This protocol is used to assay the effect of protein over-expression on fitness of E. coli . It is based on a plasmid expression of a protein of interest from an arabinose-regulated pBAD promoter followed by the measurement of the intracellular protein abundance by Western blot along with the measurement of growth parameters of E. coli cell expressing this protein.
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