Open AccessAntioxidant Activities and the In Vitro Effects on K562 Cell Viability of Flavonoids Obtained from Onion Skin by Different Extraction Methods
Author(s) -
Shi Guo-qing,
Qisen Xiang,
Zhao Wen-en,
Fan Yichao,
Tenghui Ma,
Ke Liu,
Yanqi Liu
Publication year - 2015
Publication title -
the open chemical engineering journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.2
H-Index - 10
ISSN - 1874-1231
DOI - 10.2174/1874123101509010071
Subject(s) - chemistry , extraction (chemistry) , alcohol , water extraction , dpph , chromatography , flavonoid , yield (engineering) , antioxidant , alkali metal , biochemistry , organic chemistry , materials science , metallurgy
In order to effectively extract specific flavonoids with high biological activity from onionskin, NaNO 2 -Al(NO 3 ) 3 -NaOH coloration method, spectrophotometry and MTT method were adopted to study the yield rate and contentof flavonoids obtained from alcohol water and alkali water extraction processes, the antioxidant activities and the in vitro effects on K562 cell viability. The results indicated that yield rates of flavonoids obtained by alcohol water and alkali waterextraction methods were 3.76% and 2.37%, and solid flavonoids contents were 42.20% and 76.21% respectively; underthe same concentration of total flavonoids, alkali water extract has weaker effect on removing superoxide anions andDPPH • radicals, whereas they have similar effect on removing nitrite and hydroxyl free radicals; in the test concentrationrange (40 ∼ 200 µg / mL), they have similar effect on inhibiting the proliferation activity of K562 cells; the maximum inhibitionrate of flavonoids obtained by alkali water extraction method is 77.71%, while maximum inhibition rate of flavonoidobtained by alcohol water extraction method is 70.64%. Both the flavonoids obtained from onionskins by alcoholwater extraction method and alkali water extraction method have good biological activity. The alkali water extractionmethod is simple and without any alcohol consumption, and the product purity is high. Although the yield rate and certainbiological activity of flavonoids obtained by alkali water extraction method are lower than those obtained by alcohol waterextraction method, it still has certain significance in industrial production.