Open AccessEnhanced Interferon-α2b Production in Periplasmic Space of Escherichia coli through Medium Optimization using Response Surface Method
Author(s) -
Joo Shun Tan,
Ramakrishnan Nagasundara Ramanan,
Siti Nor Ani Azaman,
Tau Chuan Ling,
M Shuhaimi,
Arbakariya Ariff
Publication year - 2009
Publication title -
the open biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.308
H-Index - 11
ISSN - 1874-0707
DOI - 10.2174/1874070700903010117
Subject(s) - periplasmic space , response surface methodology , yeast extract , escherichia coli , recombinant dna , central composite design , fermentation , chemistry , yeast , yield (engineering) , biochemistry , microbiology and biotechnology , food science , biology , chromatography , materials science , gene , metallurgy
The influence of different carbon and nitrogen sources on growth of recombinant Escherichia coli and humaninterferon-α2b (IFN-α2b) production in periplasmic space was studied in shake flask culture. A statistical method basedon Plackett-Burman design was used to screen the main medium components that greatly influenced the performance ofthe fermentation process. The optimization of medium was performed using response surface methodology (RSM) wherethree critical factors (glucose, yeast extract and peptone) were optimized using central composite design. The highest yieldof periplasmic recombinant human interferon-α2b (PrIFN-α2b) (335.8 μg/L) was predicted to be obtained in optimizedmedium containing 5.47 g/L glucose, 55.24 g/L yeast extract and 42.27 g/L peptone.. The production of IFN-α2b in periplasmicspace in optimized medium was about 2.5, 11.7 and 124.4 times higher than Terrific broth (TB), Luria-Bertani(LB), and minimal medium (M9), respectively.