Expression, Renaturation and Functional Analysis of an Excitatory Insect-Specific Toxin from Scorpion Buthus martensii Karsch | Zendy

Chong Li | Zendy; Wei Liu | Zendy; Frank Bossmans | Zendy; Rong-Huan Zhu | Zendy; Jan Tytget | Zendy; DaCheng Wang | Zendy

Open Access

Expression, Renaturation and Functional Analysis of an Excitatory Insect-Specific Toxin from Scorpion Buthus martensii Karsch

Author(s) -

Chong Li,

Wei Liu,

Frank Bossmans,

Rong-Huan Zhu,

Jan Tytget,

DaCheng Wang

Publication year - 2005

Publication title -

protein and peptide letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.432

H-Index - 58

eISSN - 1875-5305

pISSN - 0929-8665

DOI - 10.2174/0929866054696172

Subject(s) - scorpion , recombinant dna , toxin , complementary dna , denaturation (fissile materials) , sodium channel , scorpion toxin , bioassay , biochemistry , biology , escherichia coli , insect , chemistry , venom , microbiology and biotechnology , sodium , gene , botany , genetics , organic chemistry , nuclear chemistry

The cDNA of BmK IT-AP, an excitatory insect toxin from the scorpion Buthus martensi Karsch that has an analgesic effect on mammalian cells, was expressed in E. coli in the form of an inclusion body. Following denaturation and reduction, the recombinant protein was renatured and purified by liquid chromatography. The authenticity of the recombinant product was confirmed by bioassay and its electrophysiological effect on insect sodium channel.

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