Open AccessShotgun protein sequencing.
Author(s) -
JeanLoup Faulon,
Grant S. Heffelfinger
Publication year - 2009
Publication title -
osti oai (u.s. department of energy office of scientific and technical information)
Language(s) - English
Resource type - Reports
DOI - 10.2172/959081
Subject(s) - shotgun , shotgun sequencing , computational biology , sequence (biology) , dna sequencing , protein sequencing , biology , genetics , dna , peptide sequence , gene
A novel experimental and computational technique based on multiple enzymatic digestion of a protein or protein mixture that reconstructs protein sequences from sequences of overlapping peptides is described in this SAND report. This approach, analogous to shotgun sequencing of DNA, is to be used to sequence alternative spliced proteins, to identify post-translational modifications, and to sequence genetically engineered proteins
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