Open AccessIs Increased Low-dose somatic Radiosensitivity Associated with Increased Transgenerational Germline Mutation
Author(s) -
David J. Brenner
Publication year - 2008
Language(s) - English
Resource type - Reports
DOI - 10.2172/938982
Subject(s) - mutation frequency , somatic cell , bone marrow , sperm , biology , germline , germline mutation , mutation , radiosensitivity , haploinsufficiency , microbiology and biotechnology , genetics , andrology , immunology , medicine , phenotype , gene , radiation therapy
Using single-molecule polymerase chain reaction, the frequency of spontaneous and radiation-induced mutation at an expanded simple tandem repeat (ESTR) locus was studied in DNA samples extracted from sperm and bone marrow of Atm knockout (Atm+/–) heterozygous male mice. The frequency of spontaneous mutation in sperm and bone marrow in Atm+/– males did not significantly differ from that in wild-type BALB/c mice. Acute gamma-ray exposure did not affect ESTR mutation frequency in bone marrow and resulted in similar increases in sperm samples taken from Atm+/– and BALB/c males. Taken together, these results suggest that the Atm haploinsufficiency analyzed in our study does not affect spontaneous and radiation-induced ESTR mutation frequency in mice