Characterization of the mammalian DNA polymerase gene and protein. Annual progress report | Zendy

N.C. Mishra | Zendy

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Characterization of the mammalian DNA polymerase gene and protein. Annual progress report

Author(s) -

N.C. Mishra

Publication year - 1993

Publication title -

osti oai (u.s. department of energy office of scientific and technical information)

Language(s) - English

Resource type - Reports

DOI - 10.2172/90178

Subject(s) - aphidicolin , dna polymerase , dna replication , eukaryotic dna replication , dna polymerase ii , biology , polymerase , microbiology and biotechnology , genetics , dna , gene , polymerase chain reaction , reverse transcriptase

Methods were developed to purify the DNA polymerases of the {alpha}-family from Chinese hamster cells and their mutants selected as resistant to aphidicolin or specific inhibitor of DNA polymerases of the {alpha}-family. The wild type and mutant DNA polymerases were characterized with respect to their biochemical properties. A methodology was also developed to identify the replication intermediates and aphidicolin was found to inhibit a replication intermediate of the 24Kb size indicating the fact that aphidicolin inhibits the elongation process during DNA replication. This is the first demonstration of such role of aphidicolin in the eukaryotic DNA replication

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