Open AccessCharacterization of the mammalian DNA polymerase gene(s) and enzyme(s). Annual progress report
Author(s) -
N C Mishra
Publication year - 1995
Language(s) - English
Resource type - Reports
DOI - 10.2172/89557
Subject(s) - dna polymerase , microbiology and biotechnology , biology , gene , dna polymerase ii , chinese hamster ovary cell , polymerase , dna polymerase delta , dna polymerase i , dna polymerase mu , primer (cosmetics) , dna replication , dna clamp , genetics , polymerase chain reaction , chemistry , circular bacterial chromosome , cell culture , reverse transcriptase , organic chemistry
Two Genes for DNA polymerase delta were identified from the wild type Chinese hamster ovary cells. These genes were cloned via RT-PCR from mRNA prepared the Chinese hamster ovary cells using primers specific to conserved sequences of the DNA polymerase {delta} gene. The first gene encodes a PCNA dependent DNA polymerase {delta} gene whereas the second gene encodes a PCNA independent DNA polymerase {delta} gene. Methods were developed to clone these genes in expression vector and host systems. The role of the two genes in DNA replication and repair was determined