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Microbial pathways for the mobilization of mercury as Hg(O) in anoxic subsurface environments
Author(s) -
Tamar Barkay
Publication year - 2005
Publication title -
osti oai (u.s. department of energy office of scientific and technical information)
Language(s) - English
Resource type - Reports
DOI - 10.2172/893449
Subject(s) - anoxic waters , pseudomonas stutzeri , shewanella putrefaciens , serratia marcescens , microbiology and biotechnology , bacteria , pseudomonas , biology , shewanella , siderophore , mercury (programming language) , stenotrophomonas , gene , ecology , genetics , escherichia coli , computer science , programming language
The goal of our project which was initiated in June 2005 is focused on the presence of merA in microbial communities of anoxic environments and the effect of anaerobic respiratory pathways on MR expression and activities. The following progress has been made to date: PCR primers were designed to span the known phylogenetic range of merA genes of Gram-negative bacteria. In control experiments, these primers successfully amplified a 288 bp region at the 3? end of previously characterized merA genes from Shewanella putrefaciens pMERPH, Acidithiobacillus ferrooxidans, Pseudomonas stutzeri pPB, Tn5041, Pseudomonas sp. K-62, and Serratia marcescens pDU1358

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