Open AccessIDENTIFICATION AND CHARACTERIZATION OF THERMOBIFIDA FUSCA GENES INVOLVED IN PLANT CELL WALL DEGRADATION.
Author(s) -
David B. Wilson
Publication year - 2006
Language(s) - English
Resource type - Reports
DOI - 10.2172/862421
Subject(s) - gene , cellulose , gene family , hydrolase , chemistry , biochemistry , glucanase , cell wall , biology , microbiology and biotechnology , enzyme , gene expression
Micro-array experiments identified a number of Thermobifida fusca genes which were upregulated by growth on cellulose or plant biomass. Five of these genes were cloned, overexpressed in E. coli and the expressed proteins were purified and characterized. These were a xyloglucanase,a 1-3,beta glucanase, a family 18 hydrolase and twocellulose binding proteins that contained no catalytic domains. The catalyic domain of the family 74 endoxyloglucanase with a C-terminal, cellulose binding module was crystalized and its 3-dimensional structure was determined by X-ray crystallography