Open AccessFinal Report on Grant DE-FG-02ER63350
Author(s) -
John H. Miller
Publication year - 2005
Publication title -
osti oai (u.s. department of energy office of scientific and technical information)
Language(s) - English
Resource type - Reports
DOI - 10.2172/840787
Subject(s) - dna , dna glycosylase , dna damage , guanine , dna repair , bending , biophysics , molecular dynamics , chemistry , oxidative stress , biochemistry , computational biology , biology , physics , computational chemistry , gene , thermodynamics , nucleotide
Research funded by grant DE-FG-02ER63350 focused on DNA bending measured NMR spectroscopy and modeled by classical molecular dynamics (MD) simulation. Bending is a structural aspect of DNA that is plays a key role in its function. The most important finding of our research was that oxidation of guanine, a ubiquitous DNA lesion caused by endogenous and environmental oxidative stress, changes DNA bending dynamics in a way that favors binding of glycosylases, repair enzymes that remove damaged bases from DNA. Hence, the effect of 8-oxoguanine on DNA bending contributes to its recognition and removal by the base excision repair system
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