Final Technical Report for DOE Grant DE-FG02-91ER20038
Author(s) -
Timothy Nelson
Publication year - 2001
Publication title -
osti oai (u.s. department of energy office of scientific and technical information)
Language(s) - English
Resource type - Reports
DOI - 10.2172/833889
Subject(s) - photosynthesis , gene , malic enzyme , transformation (genetics) , biology , botany , genus , promoter , malate dehydrogenase , computational biology , enzyme , dehydrogenase , genetics , biochemistry , gene expression
The existence of species within the plant genus Flaveria with differing leaf cell arrangements and photosynthetic schemes (C3, C4, C3-C4) enabled us to identify genetic elements (promoters, 3'UTRs) that are responsible for the regulation of pre-existing metabolic genes in the pattern required for the high-efficiency C4 photosynthetic scheme. We analyzed DNA elements regulating the rbcS, malic enzyme, and malate dehydrogenase gene families in several species of Flaveria, using transient and stable transformation methods
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