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Genetic Engineering of a Radiation-Resistant Bacterium for Biodegradation of Mixed Wastes
Author(s) -
Mary E. Lidstrom
Publication year - 2000
Publication title -
osti oai (u.s. department of energy office of scientific and technical information)
Language(s) - English
Resource type - Reports
DOI - 10.2172/828598
Subject(s) - deinococcus radiodurans , waste management , mixed waste , biodegradation , radioactive waste , environmental science , contamination , bioremediation , genotoxicity , biochemical engineering , microbiology and biotechnology , bacteria , engineering , chemistry , biology , ecology , genetics , organic chemistry , toxicity
The mixture of toxic chemicals, heavy metals, halogenated solvents and radionuclides in many DOE waste materials presents a challenging problem for separating the different species and disposing of individual contaminants. One approach for dealing with mixed wastes is to genetically engineer the radiation-resistant bacterium, Deinococcus radiodurans to survive in and detoxify DOE's mixed waste streams, and to develop process parameters for treating mixed wastes with such constructed strains. The goal for this project is to develop a suite of genetic tools for Deinococcus radiodurans and to use these tools to construct and test stable strains for detoxification of haloorganics in mixed wastes

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