Open AccessFinal technical report. A sodium-cycle based organism with improved membrane resistance aimed at increasing the efficiency of energy biotransformations
Author(s) -
Kim Lewis
Publication year - 2001
Language(s) - English
Resource type - Reports
DOI - 10.2172/811354
Subject(s) - operon , sodium , sodium pyruvate , oxidative phosphorylation , escherichia coli , recombinant dna , biochemistry , chemistry , atpase , membrane , enzyme , gene , organic chemistry
The aim of the project was to express in E. coli components that would allow a formation of oxidative phosphorylation based on a sodium cycle. This would improve the resistance of cells to organic solvents, detergents and other toxins. The author cloned and expressed the nqr operon from H. influenzae in E. coli. Experiments with membrane vesicles indicated the presence of the functional recombinant sodium pumping NADH dehydrogenase. A gene for a hybrid E. coli/P.modestum ATPase was constructed which will enable one to co-express a sodium ATPsynthase together with a sodium NADH dehydrogenase