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Molecular genetics of the R complex of maize. Final technical report DE-FG02-86ER13627
Author(s) -
Stephen L. Dellaporta
Publication year - 2000
Language(s) - English
Resource type - Reports
DOI - 10.2172/809192
Subject(s) - transposable element , biology , gene , transposition (logic) , genetics , gene duplication , meiosis , dna transposable elements , genome , linguistics , philosophy
A molecular genetic characterization of the maize R-r complex of maize was completed during the period of support. The complex was shown to consist of two main regions: the P region, containing the r-p gene which controlled pigmentation of plant parts, and the S subcomplex, containing two rl-s genes in head-to-head orientation and a nonfunctional component termed rl-q. By examining the DNA sequences at the junction of the rl genes, the complex was shown to be derived by a series of abortive transposition events. The transposable element involved in the gene duplication and rearrangements was characterized and called doppia. Meiotic instability of the R-r complex was also characterized. Loss of P or S function was associated with several structural changes including intrachromosomal recombination and excision of a novel transposable element that appears to show instability only during meiosis