Open AccessEngineered Antibodies for Monitoring of Polynuclear Aromatic Hydrocarbons
Author(s) -
Alexander E. Karu,
Victoria A. Roberts,
Qing X. Li
Publication year - 2002
Language(s) - English
Resource type - Reports
DOI - 10.2172/791423
Subject(s) - analyte , biochemical engineering , computer science , antibody response , limiting , chemistry , computational biology , nanotechnology , antibody , engineering , materials science , chromatography , biology , mechanical engineering , immunology
This project was undertaken to fill needs in ODE's human and ecosystem health effects research, site remediation, rapid emergency response, and regulatory compliance monitoring programs. Doe has greatly stimulated development and validation of antibody-based, rapid, field-portable detection systems for small hazardous compounds. These range from simple dipsticks, microplate enzyme-linked immunosorbent assays (ELISAs), and hand-held colorimeters, to ultrasensitive microfluidic reactors, fiber-optic sensors and microarrays that can identify multiple analytes from patterns of cross-reactivity. Unfortunately, the technology to produce antibodies with the most desirable properties did not keep pace. Lack of antibodies remains a limiting factor in production and practical use of such devices. The goals of our project were to determine the chemical and structural bases for the antibody-analyte binding interactions using advanced computational chemistry, and to use this information to create useful new binding properties through in vitro genetic engineering and combinatorial library methods