Open AccessComplete Detoxification of Short Chain Chlorinated Aliphatic Compounds: Isolation of Halorespiring Organisms and Biochemical Studies of the Dehalogenating Enzyme Systems - Final Report
Author(s) -
J.M. Tiedje
Publication year - 1999
Language(s) - English
Resource type - Reports
DOI - 10.2172/775427
Subject(s) - bioremediation , biostimulation , bioaugmentation , isolation (microbiology) , biochemical engineering , detoxification (alternative medicine) , environmental chemistry , chemistry , contamination , biology , microbiology and biotechnology , ecology , engineering , medicine , alternative medicine , pathology
Work focused on the isolation and characterization of halorespiring populations, and the initial investigation of the dechlorinating enzyme systems. In addition, tools to evaluate the presence/activity to halorespiring populations in the environment were developed. The tools developed in this work (measurements of hydrogen consumption thresholds, molecular probes) are relevant for regulatory agencies in order to facilitate decisions on which bioremediation technology (biostimulation or bioaugmentation) is most promising at a particular site. In addition, a better understanding of the physiology of the halorespiring organisms as well as the biochemistry of the dehalogenating enzyme systems enhances our knowledge of how these organisms can successfully be employed in the bioremediation of contaminated sites