Library Resources for Bac End Sequencing. Final Technical Report | Zendy

Pieter J. de Jong | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Library Resources for Bac End Sequencing. Final Technical Report

Author(s) -

Pieter J. de Jong

Publication year - 2000

Publication title -

osti oai (u.s. department of energy office of scientific and technical information)

Language(s) - English

Resource type - Reports

DOI - 10.2172/772661

Subject(s) - cloning (programming) , computational biology , genomic library , library , biology , genomic dna , genetics , computer science , dna , gene , base sequence , programming language , 16s ribosomal rna

Studies directed towards the specific aims outlined for this research award are summarized. The RPCI II Human Bac Library has been expanded by the addition of 6.9-fold genomic coverage. This segment has been generated from a MBOI partial digest of the same anonymous donor DNA used for the rest of the library. A new cloning vector, pTARBAC1, has been constructed and used in the construction of RPCI-II segment 5. This new cloning vector provides a new strategy in identifying targeted genomic regions and will greatly facilitate a large-scale analysis for positional cloning. A new maleCS7BC/6J mouse BAC library has been constructed. RPCI-23 contain 576 plates (approx 210,000 clones) and represents approximately 11-fold coverage of the mouse genome

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research