Spectromicroscopy. Final report

The objectives of this project were: (1) to develop a Confocal Hyperspectral Imaging Raman Microscope (CHIRM), and (2) using modern image processing technology, to devise algorithms for hyperspectral microscopic image recovery and enhancement. The first objective involved the development of advanced techniques in confocal microscopy, spectroscopic imaging detection, and imaging processing to produce hyperspectral Raman images. The second objective was to exploit the image detectors together with advanced statistical and data handling techniques, to produce enhanced Raman spectral images and to extract the maximum amount of spectral information in the minimum time with maximum economy. In both cases the objective was to develop instrumentation and software for both diagnostic and research applications, which require maximum sensitivity and minimum complexity and cost. These objectives have been completed on time and with a reduced budget