Cloning and sequencing of the trpE gene from Arthrobacter globiformis ATCC 8010 and several related subsurface Arthrobacter isolates | Zendy

Tatyana Chernova | Zendy; V. K. Viswanathan | Zendy; Natasha E. Austria | Zendy; Brian P. Nichols | Zendy

Open Access

Cloning and sequencing of the trpE gene from Arthrobacter globiformis ATCC 8010 and several related subsurface Arthrobacter isolates

Author(s) -

Tatyana Chernova,

V. K. Viswanathan,

Natasha E. Austria,

Brian P. Nichols

Publication year - 1998

Publication title -

osti oai (u.s. department of energy office of scientific and technical information)

Language(s) - English

Resource type - Reports

DOI - 10.2172/663530

Subject(s) - arthrobacter , gene , biology , complementation , genetics , genome , sequence analysis , cloning (programming) , molecular cloning , phylogenetic tree , microbiology and biotechnology , mutant , peptide sequence , bacteria , computer science , programming language

Tryptophan dependent mutants of Arthrobacter globiformis ATCC 8010 were isolated and trp genes were cloned by complementation and marker rescue of the auxotrophic strains. Rescue studies and preliminary sequence analysis reveal that at least the genes trpE, trpC, and trpB are clustered together in this organism. In addition, sequence analysis of the entire trpE gene, which encodes component I of anthranilate synthase, is described. Segments of the trpE gene from 17 subsurface isolates of Arthrobacter sp. were amplified by PCR and sequenced. The partial trpE sequences from the various strains were aligned and subjected to phylogenetic analysis. The data suggest that in addition to single base changes, recombination and genetic exchange play a major role in the evolution of the Arthrobacter genome

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