Enzyme catalysts for a biotechnology-based chemical industry. Quarterly progress report, April 1--July 1, 1998 | Zendy

Frances H. Arnold | Zendy

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Enzyme catalysts for a biotechnology-based chemical industry. Quarterly progress report, April 1--July 1, 1998

Author(s) -

Frances H. Arnold

Publication year - 1998

Language(s) - English

Resource type - Reports

DOI - 10.2172/663431

Subject(s) - thermostability , subtilisin , esterase , biochemical engineering , microbiology and biotechnology , enzyme , catalysis , mutagenesis , chemical industry , catalytic efficiency , protein engineering , chemistry , computational biology , engineering , biochemistry , biology , organic chemistry , mutant , gene

The goal of this research is to engineer enzymes to be efficient and economically attractive catalysts for the chemical industry. The author is attempting to demonstrate generally-applicable approaches to enzyme improvement as well as develop specific catalysts for potential industrial application. Progress on three tasks are described: Random mutagenesis of pNB esterase--improved activity and stability; Directed evolution of subtilisin E to enhance thermostability; and Methods for invitro recombination

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