Genetics in methylotrophic bacteria: Appendix. Final report | Zendy

Mary E. Lidstrom | Zendy

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Genetics in methylotrophic bacteria: Appendix. Final report

Author(s) -

Mary E. Lidstrom

Publication year - 1998

Publication title -

osti oai (u.s. department of energy office of scientific and technical information)

Language(s) - English

Resource type - Reports

DOI - 10.2172/656506

Subject(s) - promoter , gene , lac operon , plasmid , biology , genetics , transcription (linguistics) , bacteria , methylobacterium , gene expression , linguistics , philosophy , 16s ribosomal rna

This research has focused primarily on promoters in Methylobacterium extorquens AM1 and in methanotrophic bacteria. In Methylobacterium extorquens work continued on the moxF promoter. The author constructed chromosomal lacZ fusions of this promoter to avoid the regulation problems of plasmid-borne fragments and has shown that this is regulated normally in the chromosome. She has constructed lacZ fusions to some of the mox genes involved in the synthesis of the cofactor, PQQ, in order to carry out similar analysis of transcription of PQQ genes. The author has continued to isolate mox genes in methanotrophs for the purpose of studying their promoters and transcriptional regulation

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