Enzyme catalysts for a biotechnology-based chemical industry. Quarterly progress report, January 1--April 1, 1998 | Zendy

Frances H. Arnold | Zendy

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Enzyme catalysts for a biotechnology-based chemical industry. Quarterly progress report, January 1--April 1, 1998

Author(s) -

Frances H. Arnold

Publication year - 1998

Publication title -

osti oai (u.s. department of energy office of scientific and technical information)

Language(s) - English

Resource type - Reports

DOI - 10.2172/656465

Subject(s) - thermostability , subtilisin , microbiology and biotechnology , esterase , biochemical engineering , enzyme , catalysis , mutagenesis , chemical industry , protein engineering , catalytic efficiency , directed evolution , chemistry , biochemistry , engineering , computational biology , biology , organic chemistry , mutant , gene

The goal of this research is to engineer enzymes to be efficient and economically attractive catalysts for the chemical industry. The author is attempting to demonstrate generally-applicable approaches to enzyme improvement as well as develop specific catalysts for potential industrial application. The research is focused on the following areas: (1) Random mutagenesis of pNB esterase: improved activity and stability; (2) Directed evolution of subtilisin E to enhance thermostability; and (3) Methods for in vitro recombination

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