Open AccessImmobilization and orientation of Photosystem I reaction centers on solid surfaces. Final report
Publication year - 1998
Language(s) - English
Resource type - Reports
DOI - 10.2172/569087
Subject(s) - guanine , dna , intercalation (chemistry) , chemistry , stereochemistry , cleavage (geology) , crystallography , nucleic acid , a dna , duplex (building) , oligonucleotide , base pair , selectivity , bond cleavage , nucleotide , photochemistry , materials science , biochemistry , gene , inorganic chemistry , fracture (geology) , composite material , catalysis
The overall objective of this project was to test the potential for immobilization and orientation of Photosystem I reaction center protein on solid surfaces. In order to maximize the resources available for this work, bleomycin complexes were used as a test substrate. The reaction of [(H{sub 2}O)(NH{sub 3}){sub 5}Ru{sup II}]{sup 2+} with bleomycin forms at least two stable products following oxidation to the Ru(III) analog. Spectroscopic and electrochemical measurements indicate monodentate binding of [(NH{sub 3}){sub 5}Ru{sup III}] to the imidazole and pyrimidine moieties, with coordination to the latter involving the exocyclic amine nitrogen. DNA cleavage studies show the complexes to be ineffective in DNA strand scission