Open AccessA molecular genetic approach to understanding eukaryotic cellulose synthesis. Final technical report, March 15, 1995--March 14, 1997
Author(s) -
Richard L. Blanton
Publication year - 1997
Language(s) - English
Resource type - Reports
DOI - 10.2172/548677
Subject(s) - cellulose , dictyostelium discoideum , insertional mutagenesis , mutagenesis , biosynthesis , mutant , chemistry , biochemistry , computational biology , biology , microbiology and biotechnology , gene
In an attempt to understand eukaryotic cellulose synthesis, this research investigates the cellulose biosynthetic pathway of Dictyostelium discoideum. Attention is focused on the following: use of a newly developed method of tagged insertional mutagenesis to generate mutants in cellulose biosynthesis; and exploiting the monolayer culture system to identify differences in mRNA populations between DIF-induced (and cellulose-synthesizing) versus non-induced cells