Open AccessPreparation of oligonucleotide arrays for hybridization studies: Final report, 2/15/92-5/4/96
Author(s) -
Michael C. Pirrung
Publication year - 1996
Publication title -
osti oai (u.s. department of energy office of scientific and technical information)
Language(s) - English
Resource type - Reports
DOI - 10.2172/486589
Subject(s) - oligonucleotide , dna , combinatorial chemistry , process (computing) , dna synthesis , chemistry , computer science , programming language , biochemistry
We have developed several novel ways to prepare DNA. In each, the deprotection step in each synthesis cycle is accomplished with light. The group we developed for this process is dimethoxybenzoin (DMB) which, when attached to acidic functionalities, is readily removed with long wavelength (350nm) UV irradiation that will not damage the DNA bases
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