Iron regulation of gene expression in the Bradyrhizobium japonicum/soybean symbiosis. Final technical report, June 1, 1991--May 31, 1995 | Zendy

Mary Lou Guerinot | Zendy

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Iron regulation of gene expression in the Bradyrhizobium japonicum/soybean symbiosis. Final technical report, June 1, 1991--May 31, 1995

Author(s) -

Mary Lou Guerinot

Publication year - 1996

Publication title -

osti oai (u.s. department of energy office of scientific and technical information)

Language(s) - English

Resource type - Reports

DOI - 10.2172/475612

Subject(s) - bradyrhizobium japonicum , gene , gene expression , symbiosis , biology , untranslated region , prime (order theory) , microbiology and biotechnology , genetics , messenger rna , rhizobiaceae , bacteria , mathematics , combinatorics

B.japonicum produces ALA in a reaction catalyzed by the product of the hemA gene. Expression of the gene is affected by iron availability. To address the question of how the 5 prime untranslated region of the hemA transcript is involved in iron regulation, evenly spaced 10bp deletions within the hemA leader region was constructed and effects on hemA-lacZ expression were determined

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