Capillary electrophoretic separation of DNA restriction fragments using dilute polymer solutions | Zendy

B. Braun | Zendy; W. Blanch | Zendy; J. M. Prausnitz | Zendy

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Capillary electrophoretic separation of DNA restriction fragments using dilute polymer solutions

Author(s) -

B. Braun,

W. Blanch,

J. M. Prausnitz

Publication year - 1997

Publication title -

osti oai (u.s. department of energy office of scientific and technical information)

Language(s) - English

Resource type - Reports

DOI - 10.2172/453768

Subject(s) - capillary electrophoresis , polyvinylpyrrolidone , polymer , electrophoresis , capillary action , dna , chromatography , chemistry , ethylene oxide , gel electrophoresis of nucleic acids , polymer chemistry , materials science , copolymer , biochemistry , organic chemistry , composite material

Because the mechanism of DNA separation in capillary electrophoresis is not well understood, selection of polymers is a {open_quotes}trial-and-error{close_quotes} procedure. We investigated dilute-solution DNA separations by capillary electrophoresis using solutions of four polymers that differ in size, shape and stiffness. Hydroxyethylcellulose of high molecular weight provides excellent separation of large DNA fragments (2027 bp - 23130 bp). Polyvinylpyrrolidone separates DNA from 72 bp to 23 kbp and star-(polyethylene oxide), like linear poly (ethylene oxide), provides separation of fragments up to 1353 bp

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