Large scale solubilization of coal and bioconversion to utilizable energy. Eleventh quarterly technical progress report, April 1, 1996--June 30, 1996 | Zendy

N.C. Mishra | Zendy

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Large scale solubilization of coal and bioconversion to utilizable energy. Eleventh quarterly technical progress report, April 1, 1996--June 30, 1996

Author(s) -

N.C. Mishra

Publication year - 1996

Publication title -

osti oai (u.s. department of energy office of scientific and technical information)

Language(s) - English

Resource type - Reports

DOI - 10.2172/419268

Subject(s) - neurospora , yeast , solubilization , clone (java method) , bioconversion , fraction (chemistry) , biochemistry , coal , chemistry , biology , chromatography , gene , organic chemistry , fermentation , mutant , neurospora crassa

Neurospora has the capability to solubilize coal and the protein fraction accounting for this ability has been isolated. During this period the cola solubilizing activity (CSA) was fractionated and partially sequenced. The activity has been determined to be a tyrosinase and/or a phenol oxidase. The amino acid sequence of the protein was used to prepare oligonucleotides to identify the clone carrying Neurospora CSA. It is intended to clone the Neurospora gene into yeast, since yeast cannot solubilize coal, to further characterize the CSA

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